A field study was carried out in a remote broadleaved Fagus longipetiolata forest in Shaanxi province, China to study the natural local
distribution of Lentinula edodes. Following spatial mapping, 24 fruit bodies were collected for tissue isolation into axenic culture. 24
genets distributed on fallen tree trunks within a distance of 120 m were identified and clustered into 7 groups using the unweighted
pair-group method algorithm using data based on colony morphologies, abilities to degrade aromatic poly-R478 dye, somatic
incompatibility reaction patterns and DNA fingerprints. Among the parameters used, the somatic incompatibility reaction, a
polygenic phenotype, was the most differentiating, identifying 22 incompatible classes. Two sets of fruit bodies of different genets
were so close together that they would otherwise have been described as aggregate fruits of presumed identical origin. Eighteen
genets found on the same 5.6 m long tree trunk divided roughly into two clusters, matching their spatial distribution, and a nearby
branch bore another distinct cluster. More heterogeneity was encountered between isolates the greater the distance separating them
on the original site. Genets on the same tree trunk showed more compatible somatic reactions among themselves, and their DNA
fingerprints showed higher similarity. Nevertheless, considering the totality of phenotypic characters, each fruit body is a genet in L.
edodes. Such features are concluded to result from a reproductive strategy which depends on basidiospore dispersal. Within each
cluster of isolates from the collection site genets seemed to have arisen from multiple sib-mating events. Thus, a cluster may
represent a lineage of L. edodes. Individualism in L. edodes is based on a strong somatic incompatibility system. Strong competition
from contaminating individuals arriving as air-borne basidiospores could explain decreased and fluctuating crop yields which are now
frequently observed in later flushes from the outdoor wood log cultivation system. Further, it would also explain why multispore
spawn is not favoured in artificial cultivation of this economically important edible mushroom.